Application of SifaInDel 45plex System in the Han and Mongolian Populations / 法医学杂志

OBJECTIVES@#To investigate the genetic polymorphism of InDel loci in SifalnDel 45plex system in the Han population in Jiangsu Province and the Mongolian population in Inner Mongolia, and to evaluate the effectiveness of the system in forensic medicine.@*METHODS@#SifaInDel 45plex system was used for genotyping in blood samples of 398 unrelated individuals from the above two populations, and allele frequencies and population genetic parameters of the two populations were calculated respectively. Eight intercontinental populations in the gnomAD database were used as reference populations. The genetic distances between the two studied populations and eight reference populations were calculated based on the allele frequencies of 27 autosomal-InDels (A-InDels). The phylogenetic trees and multidimensional scaling (MDS) analysis diagrams were constructed accordingly.@*RESULTS@#Among two studied populations, the 27 A-InDels and 16 X-InDels showed no linkage disequilibrium between each other and the allele frequency distributions were in Hardy-Weinberg equilibrium. The CDP of the 27 A-InDels in two studied populations were all higher than 0.999 999 999 9, and the CPEtrio were all less than 0.999 9. The CDP of the 16 X-InDels in Han in Jiangsu and Mongolian in Inner Mongolia female and male samples were 0.999 997 962, 0.999 998 389, and 0.999 818 940, 0.999 856 063, respectively. The CMECtrio were all less than 0.999 9. The results of population genetics showed that the Jiangsu Han nationality, Inner Mongolia Mongolian nationality and East Asian population clustered into one branch, showing closer genetic relationship. The other 7 intercontinental populations clustered into another group. And the above 3 populations displayed distant genetic relationships with the other 7 intercontinental populations.@*CONCLUSIONS@#The InDels in the SifaInDel 45plex system have good genetic polymorphism in the two studied populations, which can be used for forensic individual identification or as an effective complement for paternity identification, and to distinguish different intercontinental populations.

Effect of Epstein-Barr virus infection on the inflammatory response in the acute exacerbation of chronic obstructive pulmonary disease / 中国实用内科杂志

OBJECTIVE: To study the effect of Epstein-Barr virus(EB) virus infection on acute exacerbation of chronic obstructive pulmonary disease. METHODS: 156 patients with acute exacerbation of chronic obstructive pulmonary disease were divided into two groups, 41 patients with EB virus infection and the group 115 patients without EB virus infection.which colleceted from the Department of Respiratory and Critical Care Medicine, Guizhou Provincial People's Hospital From October 2015 to August 2017. Epstein-Barr virus(EB) DNA was detected by PCR, serum EB antibody IgG and IgM were detected by Elisa, and the expression level of PCT, CRP, IL6,IL-17 and IL-10 in serum were detected by Elisa.Sputum culture and sensitivity were used to analyze the bacteriological characteristics. The characteristics of common pathogens of pulmonary infection were also analyzed in patients with Epstein-Barr virus(EBV) infection,and the therapeutic effects and prognosis were analyzed comprehensively. RESULTS: The EBV-infected AECOPD patients had significantly higher levels of PCT, CRP and IL-6 IL-17, IL-10 in serum of patients with lung infection than those of AECOPD patients with non-EB virus infection. The etiological characteristics of EBV-infected AECOPD patients were mostly G-, The EBV-infected AECOPD patients whose the proportion of drug-resistant bacteria increased, anti-infective treatment time prolonged, complications increased, admitted to intensive care unit increased. Blood gas, the infection of Epstein-Barr virus AECOPD patients was significantly higher PaCO2, PaO2 decreased significantly, and the difference was statistically significant. However, the lung function, no significant difference between the two groups. CONCLUSION: EBV infection aggravates inflammatory response in patients with AECOPD, and promotes the progress and deterioration of the disease.